Monitoring tritrophic biocontrol interactions between Bacillus spp., Fusarium oxysporum f. sp. cubense, tropical race 4 and banana plants in vivo based on fluorescent transformation system

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dc.creatorHe, Ping
dc.creatorLi, Shu
dc.creatorXu, Shengtao
dc.creatorFan, Huacai
dc.creatorWang, Yongfen
dc.creatorZhou, Wei
dc.creatorFu, Gang
dc.creatorHan, Guangyu
dc.creatorWang, Yunyue
dc.creatorZheng, Si-Jun
dc.date2021-10-13
dc.date2021-12-10T07:42:49Z
dc.date2021-12-10T07:42:49Z
dc.date.accessioned2026-06-27T13:30:12Z
dc.descriptionBacillus spp. are effective biocontrol agents for Fusarium wilt of banana, tropical race 4 (TR4). This study explores the colonization by B. subtilis, B. velezensis and B. amyloliquefaciens of host banana plants and elucidates the mechanism of antagonistic TR4 biocontrol. The authors selected one B. subtilis strain, three B. velezensis strains and three B. amyloliquefaciens strains that are proven to significantly inhibit TR4 in vitro, optimized the genetic transformation conditions and explored their colonization process in banana plants. The results showed that we successfully constructed an optimized fluorescent electro-transformation system (OD600 of bacteria concentration = 0.7, plasmid concentration = 50 ng/μL, plasmid volume = 2 μL, transformation voltage = 1.8kV, transformation capacitance = 400Ω) of TR4-inhibitory Bacillus spp. strains. The red fluorescent protein (RFP)-labelled strains were shown to have high stability with a plasmid-retention frequency above 98%, where bacterial growth rates and TR4 inhibition are unaffected by fluorescent plasmid insertion. In vivo colonizing observation by Laser Scanning Confocal Microscopy (LSCM) and Scanning Electron Microscopy (SEM) showed that Bacillus spp. can colonize the internal cells of banana plantlets roots. Further fluorescent observation by (LSCM) showed these RFP-labelled bacteria exhibit chemotaxis (chemotaxis ratio was 1.85±0.04) towards green fluorescent protein (GFP)-labelled TR4 hyphae in banana plants. We conclude that B. subtilis, B. velezensis and B. amyloliquefaciens can successfully colonize banana plants and interact with TR4. Monitoring its dynamic interaction with TR4 and its biocontrol mechanism is under further study.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/116646
dc.identifier.urihttp://hdl.handle.net/123456789/61430
dc.languageen
dc.publisherFrontiers Media
dc.rightsOpen Access
dc.sourceHe, P.; Li, S.; Xu, S.; Fan, H.; Wang, Y.; Zhou, W.; Fu, G.; Han, G.; Wang, Y.; Zheng, S.-J. (2021) Monitoring tritrophic biocontrol interactions between Bacillus spp., Fusarium oxysporum f. sp. cubense, tropical race 4 and banana plants in vivo based on fluorescent transformation system. Frontiers in Microbiology 12: 754918 13 p. ISSN: 1664-302X
dc.subjectbiological control
dc.subjectdisease management
dc.subjectmonitoring techniques
dc.subjectcontrol biológico
dc.subjectgestión de la enfermedad
dc.subjecttécnicas de vigilancia
dc.titleMonitoring tritrophic biocontrol interactions between Bacillus spp., Fusarium oxysporum f. sp. cubense, tropical race 4 and banana plants in vivo based on fluorescent transformation system
dc.typeJournal Article

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