INVESTIGATION ON THE HAEMAGLUTINATING ACTIVITY OCCURRING IN THREE SPECIES OF LUPINS
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Instituto Nacional de Ciencias Agrícolas
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Lupin is a leguminous plant greatly used as human food and animal feed all around the world. It has several agronomic advantages related to other species of the family. In this paper, the haemagglutinating activity from seeds of three species of lupins: two Mediterraneans ( L. albus and L. angustifolius ) and one from South America ( L. mutabilis ) was studied. An important haemaglutinating activity was found in extracts from mature seeds, in each case, against rabbit erythrocytes, specially when these cells had been previously treated with a trypsine solution to partially hydrolyze their membranes. Assays were not influenced by different temperatures and similar results were observed at 25 and 4 ºC. Inhibition of haemagglutination with carbohydrates and glycoproteins was performed to determine haemagglutinin specificity; the three species tested followed a highly specific behavior per galactose, as well as per disaccharide (Melibiose) and trisaccharide (Raffinose) containing galactose monomer. Among the glycoproteins tested, it was observed a high specificity towards Porcine Mucin, Fetuin, Asialofetuin, Thyroglobulin and in a lesser extent to Ovalbumin. There was not inhibition of haemaglutination by other carbohydrates and glycoproteins tested or they did it in higher concentrations.
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