Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP)

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dc.creatorNkere, C.K.
dc.creatorOyekanmi, J.O.
dc.creatorSilva, G.
dc.creatorBömer, M.
dc.creatorAtiri, G.I.
dc.creatorOnyeka, J.
dc.creatorMaroya, N.G.
dc.creatorSeal, S.E.
dc.creatorKumar, P. Lava
dc.date2018-04
dc.date2020-07-03T08:48:56Z
dc.date2020-07-03T08:48:56Z
dc.date.accessioned2026-06-27T15:53:42Z
dc.descriptionA closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the amplification products in YMV-positive samples are visualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tuber tissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintaining the same specificity.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/108663
dc.identifier.urihttp://hdl.handle.net/123456789/116667
dc.languageen
dc.publisherSpringer
dc.rightsOpen Access
dc.sourceNkere, C.K., Oyekanmi, J.O., Silva, G., Bömer, M., Atiri, G.I., Onyeka, J., ... & Kumar, P.L. (2018). Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP). Archives of Virology, 163(4), 1057-1061.
dc.subjectyams
dc.subjectviruses
dc.subjectplant viruses
dc.subjectplant condition
dc.subjectplant diseases
dc.subjectproduction
dc.subjectvirology
dc.titleChromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP)
dc.typeJournal Article

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Instituto Internacional de Agricultura Tropical (IITA)