Behavior of Jatropha curcas L. seeds under osmotic stress: germination and cell cycle activity
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Universidade Estadual de Maringá
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Jatropha curcas is an oil-rich Euphorbiaceae seed spec ies renowned for its apparent tolerance to environmental stresses. It is considered a pro mising source of renewabl e feedstock for biodiesel production in the Brazilian semiarid region where cr op establishment requires a better understanding of the mechanisms leading to proper seed and plant beha vior under water restrictive conditions. This study describes physiological and cytological profiles of J. curcas seeds imbibed in water restriction conditions by means of osmotic stress or osmoconditioning. Seeds were characterized by size, weight, moisture content and dry mass, germinability, and cell cycle activation by means of tubulin and microtubule cytoskeleton accumulation. Osmoconditioning at -0.8 MPa did no t induce priming effects as it did not improve the physiological quality of the seed lots. Western blo tting and immunocytochemical analysis revealed an increasing accumulation of tubulin and microtubule cytoskeleton in seeds imbibed in water for 48h onwards, culminating in the onset of mitotic configurat ions after germination. Only cortical microtubules were observed during seed osmo conditioning, whereas mitotic microtubules only occurred after re- imbibition of osmoconditioned seeds in water and subsequent germination.
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Agrociencias