Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation

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dc.creatorJaramillo, Angélica M.
dc.creatorSierra, Santiago
dc.creatorChavarriaga-Aguirre, Paul
dc.creatorCastillo, Diana Katherine
dc.creatorGkanogiannis, Anestis
dc.creatorBecerra Lopez-Lavalle, Luis Augusto
dc.creatorArciniegas Vega, Juan Pablo
dc.creatorSun, Tianhu
dc.creatorLi, Li
dc.creatorWelsch, Ralf
dc.creatorBoy, Erick
dc.creatorÁlvarez, Daniel
dc.date2022-01-07
dc.date2023-02-09T14:44:36Z
dc.date2023-02-09T14:44:36Z
dc.date.accessioned2026-06-27T15:16:06Z
dc.descriptionCassava (Manihot esculenta Crantz) biofortification with provitamin A carotenoids is an ongoing process that aims to alleviate vitamin A deficiency. The moderate content of provitamin A carotenoids achieved so far limits the contribution to providing adequate dietary vitamin A levels. Strategies to increase carotenoid content focused on genes from the carotenoids biosynthesis pathway. In recent years, special emphasis was given to ORANGE protein (OR), which promotes the accumulation of carotenoids and their stability in several plants. The aim of this work was to identify, characterize and investigate the role of OR in the biosynthesis and stabilization of carotenoids in cassava and its relationship with phytoene synthase (PSY), the rate-limiting enzyme of the carotenoids biosynthesis pathway. Gene and protein characterization of OR, expression levels, protein amounts and carotenoids levels were evaluated in roots of one white (60444) and two yellow cassava cultivars (GM5309-57 and GM3736-37). Four OR variants were found in yellow cassava roots. Although comparable expression was found for three variants, significantly higher OR protein amounts were observed in the yellow varieties. In contrast, cassava PSY1 expression was significantly higher in the yellow cultivars, but PSY protein amount did not vary. Furthermore, we evaluated whether expression of one of the variants, MeOR_X1, affected carotenoid accumulation in cassava Friable Embryogenic Callus (FEC). Overexpression of maize PSY1 alone resulted in carotenoids accumulation and induced crystal formation. Co-expression with MeOR_X1 led to greatly increase of carotenoids although PSY1 expression was high in the co-expressed FEC. Our data suggest that posttranslational mechanisms controlling OR and PSY protein stability contribute to higher carotenoid levels in yellow cassava. Moreover, we showed that cassava FEC can be used to study the efficiency of single and combinatorial gene expression in increasing the carotenoid content prior to its application for the generation of biofortified cassava with enhanced carotenoids levels.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/128585
dc.identifier.urihttp://hdl.handle.net/123456789/99403
dc.languageen
dc.publisherPublic Library of Science
dc.rightsOpen Access
dc.sourceJaramillo, A.M.; Sierra, S.; Chavarriaga-Aguirre, P., Castillo, K.D.; Gkanogiannis, A.; Becerra López-Lavalle, L.A.; Arciniegas, J.P.; Sun, T.; Li, L.; Welsch, R.; Boy, E.; Álvarez, D. (2022) Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation. PLoS One 17(1): e0263168 24 p. ISSN: 1932-6203
dc.subjectprovitamins
dc.subjectcarotenoids
dc.subjectprotein requirements
dc.subjectprovitaminas
dc.subjectcarotenoides
dc.subjectrequerimientos proteicos
dc.subjectvitamin a
dc.subjectbiofortification
dc.subjecthealth
dc.subjectretinol
dc.subjectnutrition
dc.subjectvitamin deficiencies
dc.subjectcassava
dc.subjectdiet
dc.subjectpublic health
dc.titleCharacterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation
dc.typeJournal Article

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Instituto Internacional de Investigación sobre Políticas Alimentarias (IFPRI)