Characterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level.

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dc.contributorGlaucia E. O. Midorikawa, Universidade de Brasília (UnB)
dc.contributorMARIA DE LOURDES MENDES DE SOUZA, CTAA
dc.contributorOTNIEL FREITAS SILVA, CTAA
dc.contributorJUREMA DO SOCORRO AZEVEDO DIAS, CPAF-AP
dc.contributorLuis I. B. Kanzaki, Universidade de Brasília (UnB)
dc.contributorRogerio E. Hanada, Instituto Nacional de Pesquisas da Amazônia (Inpa)
dc.contributorRenata M. L. C. Mesquita, Instituto Nacional de Pesquisas da Amazônia (Inpa)
dc.contributorRIVADALVE COELHO GONCALVES, CPAF-AC
dc.contributorVIRGINIA DE SOUZA ALVARES, CPAF-AC
dc.contributorDANIELA MATIAS DE C BITTENCOURT, CPAA
dc.contributorRobert N. G. Miller, Universidade de Brasília (UnB).
dc.creatorMIDORIKAWA, G. E. O.
dc.creatorSOUZA, M. de L. M. de
dc.creatorSILVA, O. F.
dc.creatorDIAS, J. do S. A.
dc.creatorKANZAKI, L. I. B.
dc.creatorHANADA, R. E.
dc.creatorMESQUITA, R. M. L. C.
dc.creatorGONCALVES, R. C.
dc.creatorALVARES, V. de S.
dc.creatorBITTENCOURT, D. M. de C.
dc.creatorMILLER, R. N. G.
dc.date2014-06-02T11:11:11Z
dc.date2014-06-02T11:11:11Z
dc.date2014-06-02
dc.date2014
dc.date2019-01-09T11:11:11Z
dc.date.accessioned2026-06-30T22:48:21Z
dc.descriptionBrazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius and A. flavus observed on Brazil nut, and associated risk of mycotoxin accumulation, simple identification methods for such mycotoxigenic species are of importance for Hazard Analysis Critical Control Point system implementation. The assay for the genus Aspergillus represents progress towards specific PCR identification and detection of mycotoxigenic species.
dc.identifierBMC Microbiology, v.14, n. 138, 2014.
dc.identifier1471-2180
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/987473
dc.identifier10.1186/1471-2180-14-138
dc.identifier.urihttp://hdl.handle.net/123456789/368464
dc.languageeng
dc.rightsopenAccess
dc.subjectCastanha do brasil
dc.subjectEnfermedades y desórdenes de las plantas
dc.subjectNuez del Brasil
dc.subjectIdentificación de patógenos
dc.subjectDoença de planta
dc.subjectCastanha do pará
dc.subjectBertholletia excelsa
dc.subjectFungo
dc.subjectIdentificação
dc.subjectAspergillus flavus
dc.subjectAflatoxina
dc.subjectPlant diseases and disorders
dc.subjectBrazil nuts
dc.subjectFungi
dc.subjectPathogen identification
dc.subjectAmazonia
dc.titleCharacterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level.
dc.typeArtigo de periódico

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