Crystallization of Ranasmurfin, a blue-coloured protein from Polypedates leucomystax.

MCMAHON, S. A.; WALSH, M. A.; CHING R. T. Y.; CARTER, L. G.; DORWARD, M.; JOHNSON, K. A.; LIU, H.; OKE, M.; BLOCH JUNIOR, C.; KENNEDY, M. W.; LATIFF, A. A.; COOPER, A.; TAYLOR, G. L.; WHITE, M. F.; NAISMITH, J. H.

Crystallization of Ranasmurfin, a blue-coloured protein from Polypedates leucomystax.

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Descripción

Ranasmurfin, a previously uncharacterized ~13 kDa blue protein found in the nests of the frog Polypedates leucomystax, has been purified and crystallized. The crystals are an intense blue colour and diffract to 1.51 A˚ with P21 symmetry and unit-cell parameters a = 40.9, b = 59.9, c = 45.0 A˚ , beta = 93.3. Self-rotation function analysis indicates the presence of a dimer in the asymmetric unit. Biochemical data suggest that the blue colour of the protein is related to dimer formation. Sequence data for the protein are incomplete, but thus far have identified no model for molecular replacement. A fluorescence scan shows a peak at 9.676 keV, indicating that the protein binds zinc and suggesting a route for structure solution.