Osmolaridade ideal para execução do teste hiposmótico no sêmen in natura de ovinos (Ovis aries).

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dc.contributorALEXANDRE ROSSETTO GARCIA, CPPSE; Ana Beatriz Bossois Moura, UFF; Narian Romanello, USP; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandão, UFF; Messy Hannear de Andrade Pantoja, USP; Andréa do Nascimento Barreto, UFPA; Marco Antonio de Paula Sousa, UFPA; Daniela Botta, UFPA; Alessandro Giro, UFPA.
dc.creatorGARCIA, A. R.
dc.creatorMOURA, A. B. B.
dc.creatorROMANELLO, N.
dc.creatorFONSECA, J. F. da
dc.creatorBRANDÃO, F. Z.
dc.creatorPANTOJA, M. H. de A.
dc.creatorBARRETO, A. do N.
dc.creatorSOUSA, M. A. de P.
dc.creatorBOTTA, D.
dc.creatorGIRO, A.
dc.date2019-12-12T18:17:04Z
dc.date2019-12-12T18:17:04Z
dc.date2019-12-12
dc.date2019
dc.date2020-01-07T11:11:11Z
dc.date.accessioned2026-07-07T05:28:01Z
dc.descriptionThe study aimed to establish the most suitable osmolarity range of the hypoosmotic solution for testing the membrane integrity in raw semen of rams. Sodium citrate and fructose based solutions (S) with the following osmolarities (mOsm/L) were used: 50 (S1), 75 (S2), 100 (S3), 125 (S4), 150 (S5), 175 (S6), 200 (S7), 250 (S8), 290 (S9) and 300 mOsm/L (S10). Nine mature Santa Ines rams (21.5+0.5 months; 72.9+1.6 kg) had semen collected one month apart. After the hypoosmotic swelling test (HOST) the ejaculates were evaluated using a phase-contrast microscope. A total of 200 spermatozoa were counted and sperm cells were classified as spermatozoa with coiled tails (CD, %), strongly coiled tail (CFD, %) or non-coiled tails. According to CD, S1, S2, S3, S4, S5, S6 and S7 were superior to S8, S9 and S10 (P<0,05). According to CED, S2, S3, S4, S5, S6 and S7 were superior to S8, S9 and S10 (P<0,05). Results suggest that the 75 up to 200 mOsm/L solution would be bestfor using in HOST when raw semen of rams is analyzed.
dc.description7 e 8 de junho de 2019.
dc.formatp.160-163.
dc.identifierIn: REUNIÃO ANUAL DA ASSOCIAÇÃO BRASILEIRA DE ANDROLOGIA ANIMAL (ABRAA), 4., 2019, Goiânia. Anais... Goiânia: Editora UFMS, 2019.
dc.identifier978-85-0000-000-0
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1116740
dc.identifier.urihttp://hdl.handle.net/123456789/492138
dc.languagepor
dc.rightsopenAccess
dc.subjectSolução hiposmótica
dc.subjectHypoosmotic solution
dc.subjectRam
dc.subjectSperm
dc.subjectOvino
dc.subjectEspermatozóide
dc.titleOsmolaridade ideal para execução do teste hiposmótico no sêmen in natura de ovinos (Ovis aries).
dc.typeArtigo em anais e proceedings

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