CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding

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dc.creatorTripathi, J.N.
dc.creatorNtui, V.O.
dc.creatorRon, M.
dc.creatorMuiruri, S.K.
dc.creatorBritt, A.
dc.creatorTripathi, L.
dc.date2019
dc.date2020-03-24T10:18:23Z
dc.date2020-03-24T10:18:23Z
dc.date.accessioned2026-06-27T16:10:10Z
dc.descriptionPresence of the integrated endogenous banana streak virus (eBSV) in the B genome of plantain (AAB) is a major challenge for breeding and dissemination of hybrids. As the eBSV activates into infectious viral particles under stress, the progenitor Musa balbisiana and its derivants, having at least one B genome, cannot be used as parents for crop improvement. Here, we report a strategy to inactivate the eBSV by editing the virus sequences. The regenerated genome-edited events of Gonja Manjaya showed mutations in the targeted sites with the potential to prevent proper transcription or/and translational into functional viral proteins. Seventy-five percent of the edited events remained asymptomatic in comparison to the non-edited control plants under water stress conditions, confirming inactivation of eBSV into infectious viral particles. This study paves the way for the improvement of B genome germplasm and its use in breeding programs to produce hybrids that can be globally disseminated.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/107838
dc.identifier.urihttp://hdl.handle.net/123456789/124399
dc.languageen
dc.publisherSpringer
dc.rightsOpen Access
dc.sourceTripathi, J.N., Ntui, V.O., Ron, M., Muiruri, S.K., Britt, A. & Tripathi, L. (2019). CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding. Communications Biology, 2(1), 1-11.
dc.subjectbananas
dc.subjectmusa
dc.subjectdna
dc.subjectbreeding
dc.subjectviruses
dc.subjectplantains
dc.subjectgenomes
dc.titleCRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding
dc.typeJournal Article

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Instituto Internacional de Agricultura Tropical (IITA)