ISOLATION OF GENOMIC DNAs FROM THE TROPICAL FRUIT TREES AVOCADO, COCONUT, GUAVA AND MANGO FOR PCR-BASED DNA MARKER APPLICATION

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dc.creatorIsis M. Ramírez
dc.creatorN. N. Rodríguez
dc.creatorJuliette Valdés-Infante
dc.creatorMaricela Capote
dc.creatorD. Becker
dc.creatorW. Rohde
dc.date2004
dc.date.accessioned2026-07-07T04:26:11Z
dc.descriptionWith tropical fruit trees, the isolation of genomic DNA with sufficient quality for the application of PCR-based DNA marker technology very often has severe problems due to the presence of inhibitors such as polysaccharides, which inhibit enzymatic DNA processing or polyphenols as inhibitors of PCR reactions. Here, different protocols for DNA extraction and purification were tested with the four tropical fruit trees guava ( Psidium guajava L.), avocado ( Persea americana Mill.), mango ( Mangifera indica L.) and coconut ( Cocos nucifera L.). The well-established CTAB protocol of Doyle and Doyle yielded excellent DNA templates for PCR amplification with mango and coconut, but not so with guava and avocado. For these latter species, several other extraction protocols also yielded only non-satisfactory results. Modification of the CTAB method with respect to CTAB bu- ffer composition and in combination with reversible adsorption to NucleoSpin columns alleviated the problems encountered with the genomic DNA of both species. The quality of DNA prepared by this procedure allowed AFLP, SSR and ISTR DNA marker analyses in guava and/or avocado.
dc.formatapplication/pdf
dc.identifier1819-4087
dc.identifierhttps://www.redalyc.org/articulo.oa?id=193230179006
dc.identifier.urihttp://hdl.handle.net/123456789/461637
dc.languageen
dc.publisherInstituto Nacional de Ciencias Agrícolas
dc.relationhttp://www.redalyc.org/revista.oa?id=1932
dc.rightsCultivos Tropicales
dc.sourceCultivos Tropicales (Cuba) Num.1 Vol.25
dc.subjectAgrociencias
dc.titleISOLATION OF GENOMIC DNAs FROM THE TROPICAL FRUIT TREES AVOCADO, COCONUT, GUAVA AND MANGO FOR PCR-BASED DNA MARKER APPLICATION
dc.typeartículo científico

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