Presence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers.

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dc.contributorSHIRLEI SCRAMIN, CNPMA
dc.contributorANAMARIA FERREIRA MAYER DENTZIEN, CNPMA
dc.contributorCESAR ROBERTO SILVA DE ROSSO, CNPMA
dc.contributorITAMAR SOARES DE MELO, CNPMA.
dc.creatorSCRAMIN, S.
dc.creatorROQUE, M. R. A.
dc.creatorDENTZIEN, A. F. M.
dc.creatorROSSO, C. R. S. de
dc.creatorMELO, I. S. de
dc.date2019-05-08T00:35:29Z
dc.date2019-05-08T00:35:29Z
dc.date1998-05-26
dc.date1998
dc.date2019-05-08T00:35:29Z
dc.date.accessioned2026-07-07T05:06:52Z
dc.descriptionTwo Acinetobacter spp. strains, denominated D-12 and D-16 (extracted from Diuron TM treated sugarcane rhizosphere) were grown in different substrates in order to determine whether catechol 1,2-dioxygenase (pyrocatechase) and catechol 2,3-dioxygenase (metapyrocatechase) are constitutive or induced enzymes. Extracellular enzymes were also measured. Although biomass was measured, the conditions required for best production of microorganisms were not established. Five different inducers were added to culture media: glucose, sodium benzoate, Diuron TM, dichloroaniline and Diuron TM plus glucose. Both strains were harvested by centrifugation and disrupted by sonication for intracellular enzyme extraction. Extracellular enzyme assays were carried out with filtered supernatant. Both strains showed significant catechol 1,2-dioxygenase activity when sodium benzoate was added, but not with other inducers. Significant manganese (MnP) and lignin (LiP) peroxidase activity was observed in all supernatants, except for D-16 in which LiP was not detected with sodium benzoate. Data obtained showed evidence of the inductive nature of catechol 1,2-dioxygenase.
dc.formatp.16
dc.identifierIn: LATIN AMERICAN BIODEGRADATION & BIODETERIORATION SYMPOSIUM, 3., 1998, Florianopolis. Resumos. Florianopolis: UFSC, 1998.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/12899
dc.identifier.urihttp://hdl.handle.net/123456789/480792
dc.languageeng
dc.rightsopenAccess
dc.subjectExtracellular enzymes
dc.subjectAcinetobacter spp
dc.titlePresence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers.
dc.typeResumo em anais e proceedings

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