Report on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistance

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dc.creatorEspitia, Paula A.
dc.creatorHernández, Luis Miguel
dc.creatorVillegas, Daniel Mauricio
dc.creatorArango, Jacobo
dc.creatorCastiblanco, Valheria
dc.date2021-09
dc.date2021-11-08T11:26:12Z
dc.date2021-11-08T11:26:12Z
dc.date.accessioned2026-06-27T13:35:49Z
dc.descriptionSpittlebugs (Hemiptera: Cercopidae) are the major production limitation for Brachiaria grasses (Urochloa spp.) in the tropical America. Incorporate host-plant resistance to new hybrids is the most cost-effective and sustainable solution to overcome the great losses caused by this key pest. Identifying regions in Urochloa genome associated with resistance to Aeneolamia varia nymphal attack, would allow to develop genotyping tools (e.g., molecular markers) that contribute to the hybrid’s selection in Urochloa interspecific breeding pipeline. Leaf tissue was extracted from 315 genotypes from the three biparental populations constructed for QTL analysis. These samples are kept at -20ºC, to be lyophilized and shipped for genotyping through RAD sequencing.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/115879
dc.identifier.urihttp://hdl.handle.net/123456789/64370
dc.languageen
dc.publisherBioversity International and the International Center for Tropical Agriculture
dc.rightsOpen Access
dc.sourceEspitia, P.; Hernández L.M.; Villegas, D.; Arango, J.; Castiblanco, V. (2021) Report on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistance. Cali (Colombia): Alliance of Bioversity and CIAT. 5 p.
dc.subjectforage
dc.subjectsampling
dc.subjectgenotypes
dc.subjectdna
dc.subjectpest resistance
dc.subjectforrajes
dc.subjectmuestreo
dc.subjectgenotipos
dc.subjectresistencia a las plagas
dc.subjectadn
dc.subjectcercopidae
dc.titleReport on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistance
dc.typeReport

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