Effect of cryopreservation on growth of different elite embryogenic cell lines of norway spruce (Picea Abies (L.) Karst.)

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dc.creatorDedicova, Beata
dc.creatorNilsson, O
dc.creatorEgertsdotter, U
dc.date2011
dc.date2014-09-24T08:41:35Z
dc.date2014-09-24T08:41:35Z
dc.date.accessioned2026-06-27T14:47:32Z
dc.descriptionCallus from seven different elite embryogenic cell lines of Norway spruce originated from the controlled crossing conducted by Skogforsk (Forest Research Institute of Sweden) have been cryopreserved in liquid nitrogen using the slow freezing method with sorbitol (0.2 and 0.4 M) and DMSO (5% v/v) as cryoprotectants. Cooloing of samples was performed in a programmable freezer Cryo Med 7452. The cryovials were inserted at +4°C and frozen at -0.3°C/min to -16°C. After 15 min at -16°C, to avoid rapid cooling and crystal formation in the cells, the cryovials were further cooled to -35°C at -0.3°C/min. At the end of the freezing program the cryovials were transferred and stored in a CBS V1500 liquid nitrogen storage unit at -196°C. After thawing and plating on proliferation medium, the recovered cells showed a short lag phase, after which they continued to grow. Lags in growth were also observed after the transfer to pre-maturation and maturation media. Somatic embryo maturation and plantlet regeneration occurred in all the tested embryogenic lines.
dc.identifierhttps://hdl.handle.net/10568/43075
dc.identifier.urihttp://hdl.handle.net/123456789/88551
dc.languageen
dc.rightsLimited Access
dc.sourceDedicova, B., Nilsson, O. and Egertsdotter, U. 2011. effect of cryopreservation on growth of different elite embryogenic cell lines of Norway spruce (Picea abies (l.) karst.). Acta Hort. (ISHS) 908:203-206 http://www.actahort.org/books/908/908_24.htm
dc.subjectcryopreservation
dc.subjectsomatic embryogenesis
dc.subjectpicea abies
dc.subjectcriopreservación
dc.subjectembriogénesis somática
dc.titleEffect of cryopreservation on growth of different elite embryogenic cell lines of norway spruce (Picea Abies (L.) Karst.)
dc.typeJournal Article

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