Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoides SC through experimental infection in cattle

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dc.creatorSchnee, C.
dc.creatorHeller, M.
dc.creatorJores, Joerg
dc.creatorTomaso, H.
dc.creatorNeubauer, N.
dc.date2011-08-12
dc.date2012-02-03T08:23:43Z
dc.date2012-02-03T08:23:43Z
dc.date.accessioned2026-06-27T17:06:00Z
dc.descriptionMycoplasma mycoides subsp. mycoides SC is the pathogenic agent of contagious bovine pleuropneumonia (CBPP), the most important disease of cattle in Africa causing significant economic losses. The re-emergence of CBPP in Europe in the 1980s and 1990s illustrates that it is still a threat also to countries that have successfully eradicated the disease in the past. Nowadays, probe-based real-time PCR techniques are among the most advanced tools for a reliable identification and a sensitive detection of many pathogens, but only few protocols have been published so far for CBPP diagnosis. Therefore we developed a novel TaqMan®-based real-time PCR assay comprising the amplification of two independent targets (MSC_0136 and MSC_1046) and an internal exogenous amplification control in a multiplex reaction and evaluated its diagnostic performance with clinical samples.
dc.identifierhttps://hdl.handle.net/10568/16413
dc.identifier.urihttp://hdl.handle.net/123456789/142172
dc.languageen
dc.publisherSpringer
dc.rightsOpen Access
dc.sourceSchnee, C., Heller, M., Jores, J., Tomaso, H., & Neubauer, H. (2011). Assessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoides SC through experimental infection in cattle. BMC Veterinary Research, 7(1), 47. https://doi.org/10.1186/1746-6148-7-47
dc.subjectdiagnosis
dc.subjectanimal diseases
dc.subjectcattle
dc.subjectlivestock
dc.titleAssessment of a novel multiplex real-time PCR assay for the detection of the CBPP agent Mycoplasma mycoides subsp. mycoides SC through experimental infection in cattle
dc.typeJournal Article

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