Interaction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta).

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dc.contributorNATONIEL FRANKLIN DE MELO, CPATSA.
dc.creatorAMBROSIO, S. T.
dc.creatorMELO, N. F. de
dc.date2017-08-04T23:46:24Z
dc.date2017-08-04T23:46:24Z
dc.date2004-09-07
dc.date2004
dc.date2018-09-11T11:11:11Z
dc.date.accessioned2026-07-07T05:47:42Z
dc.descriptionInteraction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta)). The present work reports the effect of different pH and sucrose concentrations on in vitro propagation of Nephrolepis biserrata. Fronds aseptically obtained from stolon segment culture were cultivated in MS semi-solid medium supplemented with 0, 15, 30, 45 and 60 g.L -1 sucrose and pH adjusted to 3, 5, 7 and 9, in a factorial design. Frond number and length, pinnae number, raquis length and diam eter, fresh and dry matter weight were measured. Inhibition of shoot and leaf regeneration was observed in all the pH treatments in the abs ence of sucrose. On the other hand, when sucrose was added to the medium, the shoot number increased, reaching the maximum average values of 51.25 and 38.25 shoot per explant at pH 5 and 7, respectively. Sucrose concentrations from 15 to 45g.L -1 increased leaf length and diameter and the pH 9 did not affect the dry matter weight, and was still not adequate for development of new fronds. Young sporophytes were successfully acclimated.
dc.identifierActa Botanica Brasilica, São Paulo, v. 18, n. 4, p. 809-813, 2004.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/154317
dc.identifier.urihttp://hdl.handle.net/123456789/500060
dc.languageeng
dc.rightsopenAccess
dc.subjectPteridófitas
dc.subjectCultivo in vitro
dc.subjectCultura de Tecido
dc.subjectPlanta Ornamental
dc.subjectOrnamental plants
dc.subjectTissue culture
dc.subjectNephrolepis biserrata
dc.titleInteraction between sucrose and pH during in vitro culture of Nephrolepis biserrata (Sw.) Schott (Pteridophyta).
dc.typeArtigo de periódico

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