Polymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon.

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dc.contributorC. S. SILVA, UNIVERSIDADE FEDERAL DO PARÁ
dc.contributorE. SILVA FILHO, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA
dc.contributorA. S. MATOS, UNIVERSIDADE FEDERAL DO PARÁ
dc.contributorA. S. SCHIERHOLT, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA
dc.contributorMARIA ROSA TRAVASSOS DA ROSA COSTA, CPATU
dc.contributorL. C. MARQUES, UNIVERSIDADE DA AMAZÔNIA
dc.contributorJ. S. COSTA, UNIVERSIDADE FEDERAL DO PARÁ
dc.contributorRODRIGO LIMA SALES, CPATU
dc.contributorMARIVALDO RODRIGUES FIGUEIRO, CPATU
dc.contributorJOSE RIBAMAR FELIPE MARQUES, CPATU.
dc.creatorSILVA, C. S.
dc.creatorSILVA FILHO, E.
dc.creatorMATOS, A. S.
dc.creatorSCHIERHOLT, A. S.
dc.creatorCOSTA, M. R.
dc.creatorMARQUES, L. C.
dc.creatorCOSTA, J. S.
dc.creatorSALES, R. L.
dc.creatorFIGUEIRÓ, M. R.
dc.creatorMARQUES, J. R. F.
dc.date2016-09-21T11:11:11Z
dc.date2016-09-21T11:11:11Z
dc.date2016-09-21
dc.date2016
dc.date2017-02-20T11:11:11Z
dc.date.accessioned2026-06-30T23:43:06Z
dc.descriptionWater buffaloes (Bubalus bubalis) are quite well adapted to climatic conditions in the Amazon, and in this biome, they are noted for the considerable amount of meat and milk they produce and how hard they are able to work. Because of a lack of research dedicated to improving the rearing of buffaloes in the Amazon, the objective of this study was to genetically characterize the Murrah and Mediterranean breeds, as well as a mixed-breed population, based on polymorphisms in the diacylglycerol O-acyltransferase 1 gene (DGAT1), and associate the genotypes with milk production. By using the polymerase chain reaction-single-strand conformation polymorphism technique, the alleles A (0.79), B (0.20), and D (0.01) were found in the Murrah breed. In the Mediterranean and mixed-breed buffaloes, we found alleles A (0.69) and (0.77) and B (0.31) and (0.23), respectively. The Murrah breed had the genotypes AA (0.63), AB (0.29), BB (0.05), and AD (0.03), and the Mediterranean and mixed-breed buffaloes had the genotypes AA (0.44) and (0.61), AB (0.50) and (0.31), and BB (0.06) and (0.08), respectively. For the Murrah, Mediterranean, and mixedbreed buffaloes, respectively, the expected heterozygosity values were 0.34, 0.43, and 0.35, the inbreeding coefficients were 0.78, -0.15, and 0.17, and the Hardy-Weinberg probabilities were 0.70, 0.67, and 0.52. The genotypes evaluated did not have an effect on milk production; however, the single nucleotide polymorphisms can be used in studies on genetic variability.
dc.identifierGenetics and Molecular Research, v. 15, n. 3, 2016.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1053197
dc.identifierhttp://dx.doi.org/10.4238/gmr.15038720
dc.identifier.urihttp://hdl.handle.net/123456789/388686
dc.languageeng
dc.rightsopenAccess
dc.subjectBubalino
dc.subjectBúfalo
dc.subjectLeite
dc.subjectGenótipo
dc.titlePolymorphisms in the DGAT1 gene in buffaloes (Bubalus bubalis) in the Amazon.
dc.typeArtigo de periódico

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