Isolation and typing of Clostridium spp.16S rRNA from soil samples obtained in areas with sudden mortality history in Colombia

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dc.audienceInvestigador
dc.coverageC.I Tibaitatá
dc.coverageColombia
dc.creatorOrtiz Ortega, Diego
dc.creatorVillamil Jiménez, Luis Carlos
dc.creatorRodrigo Martínez, S.
dc.date2025-06-13T19:13:08Z
dc.date2025-06-13T19:13:08Z
dc.date2012-04
dc.date2012
dc.date.accessioned2026-06-27T04:37:08Z
dc.descriptionA Longitudinal epidemiologic study was developed for the isolation, biochemical characterization and molecular typing the bacterial pathogen Clostridium spp found in soil from areas affected by bovine sudden mortality. Weincluded 10 herds, located in three localities. The genetic variability of Clostridium genus was analyzed by DNA sequence of a 1500 bp fragment from the 16S rRNA gene. Twenty four Clostridium isolates were biochemically classified as Clostridium sordellii (41,7%), Clostridium glycolicum (12,5%), Clostridium hastiforme (12,5%); Clostridium botulinum (8,3%), Clostridium butyricum (8,3%); Clostridium chauvoei (4,3%),Clostridium limosum (4,3%), Clostridium septicum (4,3%) and Clostridium tertium (4,3%). The bacteria that showed pathogenic activity were studied further by 16S rRNA gene sequencing at, which 55.5% was classified as C. botulinum, where the native strain isolated from areas with outbreaks wasfound in a different group from Clostridium spp. used by commercial laboratories for vaccine production. Additionally, the native strain identified here differs from others reported in Gen Bank, indicating that the native pathogenic Clostridium spp. is genetically different to other Clostridium spp. used to prepare immunogens affecting vaccine efficiency. Our results indicate that the use of native strains could improve commercial vaccine preparations, increasing bovine immune response.
dc.descriptionGanadería bovina
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dc.formatapplication/pdf
dc.identifierhttps://www.semanticscholar.org/paper/Isolation-and-typing-of-Clostridium-spp-.-16-S-rRNA-Ortega-Jimenez/87b22bfff9ea0b867b471ff1a9c7819cd7a3eb35?sort=relevance
dc.identifier2315-5018
dc.identifierhttp://hdl.handle.net/20.500.12324/40992
dc.identifierreponame:Biblioteca Digital Agropecuaria de Colombia
dc.identifierinstname:Corporación colombiana de investigación agropecuaria AGROSAVIA
dc.identifier.urihttp://hdl.handle.net/123456789/33235
dc.languageeng
dc.publisherSemantic Scholar
dc.relationGlobal Advanced Research Journal of Microbiology
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dc.rightsAtribución-NoComercial-CompartirIgual 4.0 Internacional
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.sourceGlobal Advanced Research Journal of Microbiology; Vol. 1, Núm. 3 (2012): Global Advanced Research Journal of Microbiology (Abri.);p. 33 - 40.
dc.subjectGenética y mejoramiento animal - L10
dc.subjectAnálisis del suelo
dc.subjectVariación genética
dc.subjectMortalidad del ganado
dc.subjectADN
dc.subjectGanadería y especies menores
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_7198
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_15975
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_92a58d79
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_2347
dc.thumbnailhttps://repository.agrosavia.co/bitstreams/98d51c8d-80f5-4f00-b470-144a252491c8/download
dc.titleIsolation and typing of Clostridium spp.16S rRNA from soil samples obtained in areas with sudden mortality history in Colombia
dc.titleIsolation and typing of Clostridium spp.16S rRNA from soil samples obtained in areas with sudden mortality history in Colombia
dc.typeArtículo científico

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