Methodological aspects in ruminal digestion studies. I. Effect of feeding frequency.
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Two experiments were conducted to compare different feeding frequencies with regard to dry matter (DM) intake, rumen contents, appaxefit ruminal retention time of the neutral detergent fiber (rNDF) and 48-h in situ DM digestibility (ISDMD). In experiment 1 three rumen-fistulated steers were fed a ration of brachiaria (Brachiaria decumbens) hay, freshly cut pigeon pea (Cajanus cajan) forage and ground sorghum; six treatments were applied in an unbalanced design: a) Hourly feeding, b) Feeding every 3 h, c) Feeding every 6 h, d) Feeding every 12 h, e) Feeding every 3 h from 6:00 AM to 9:00 PM ar which time the rest of the daily ration was given, and f) Feeding every 3 h from 6:00 AM to 6:00 PM when the rest of the daily ration was offered. In experiment 2, six animals were used to compare hourly feeding vs. feeding every 6 h in a switchback design. The ration consisted of sugarcane tops, pigeon pea forage and ground corn cars. Means for net DM ' intake (kg/ 100 kg LW/ day), rumen contents (kg DM/100 kg LW), rNDF (h), brachiaria ISDMD (%) and pigeon pea forage ISDMD (%) for experiment 1 were: 1.93, 1.76, 23,8, 66.0 and 63.1, respectively, with no significant dif`ferences. In experiment 2, the means for DM intake, rumen conterits, rNDF and pigeon pea ISDMD were: 1.76, 1.79, 37.6 and 514, respectively, again with no significant treatment differences. lt was concluded that feeding frequency is not a factor of importance in the methodology for rumen digestion studies.
Título em português: Aspectos metodológicos em estudos de digestão ruminal. I. Efeito da frequência de alimentação.
Título em português: Aspectos metodológicos em estudos de digestão ruminal. I. Efeito da frequência de alimentação.
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rumen contents, in situ digestibility, ruminal retention time, feed intake, steers