Development and validation of SSR markers for Coffea arabica L.

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dc.contributorROBSON FERNANDO MISSIO, UNIVERSIDADE FEDERAL DE VIÇOSA; EVELINE TEIXEIRA CAIXETA, SAPC; EUNIZE MACIEL ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA; LAÉRCIO ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA; NEY SUSSUMU SAKIYAMA, UNIVERSIDADE FEDERAL DE VIÇOSA.
dc.creatorMISSIO, R. F.
dc.creatorCAIXETA, E. T.
dc.creatorZAMBOLIM, E. M.
dc.creatorZAMBOLIM, L.
dc.creatorSAKIYAMA, N. S.
dc.date2011-04-09T22:27:59Z
dc.date2011-04-09T22:27:59Z
dc.date2011-03-10
dc.date2009
dc.date2011-04-10T11:11:11Z
dc.date.accessioned2026-07-01T00:24:42Z
dc.descriptionWith the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences. Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of 417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were more frequent than trinucleotides. Four repeat sequences, (AG/CT)n, (AC/GT)n, (AAG/CTT)n, and (AGG/CCT)n represented 61.1% of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSR markers were validated for further genetic studies of C. arabica.
dc.identifierIn.: CROP BREEDING AND APPLIED BIOTECHNOLOGY. v.9, p.361-371, 2009.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/880403
dc.identifier.urihttp://hdl.handle.net/123456789/403334
dc.languageeng
dc.rightsopenAccess
dc.subjectCoffee
dc.subjectSSR marker
dc.subjectEnriched genomic library
dc.subjectMolecular marker
dc.titleDevelopment and validation of SSR markers for Coffea arabica L.
dc.typeArtigo de periódico

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