Re-evaluation of Yam Mosaic Virus (YMV) detection methods

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dc.creatorEni, A.
dc.creatorHughes, J.
dc.creatorAsiedu, Robert
dc.creatorRey, M.
dc.date2012
dc.date2017-02-13T13:41:36Z
dc.date2017-02-13T13:41:36Z
dc.date.accessioned2026-06-27T16:07:07Z
dc.descriptionAccurate and timely detection is vital for mitigation of tuber yield losses resulting from yam mosaic virus (YMV) infection on yam, a major food security crop in West Africa. The observation, from our previous studies, that the triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), the most commonly used detection method for YMV, detected the virus in significantly less leaf samples than immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) necessitated a re-evaluation of YMV detection methods. In the present study, eighteen previously tested YMV positive leaf samples from Benin and Ghana were re-tested using TAS-ELISA, Protein A-sandwich (PAS) ELISA and IC-RT-PCR. Three sap dilutions, 1/10, 1/50 and 1/100, were tested for each sample. Both at 1/10 and 1/50 dilutions, PAS-ELISA and IC-RT-PCR detected YMV in 11 (61.1%) and 12 (66.7%) of the leaves respectively. Virus detection by PAS-ELISA reduced to 50% at 1/100 sap dilution and increased to 77.8% in IC-RT-PCR. YMV detection by TAS-ELISA varied between 38.9% and 16.7% at 1/10 and 1/100 dilutions respectively. These results indicate a deficiency in the use of TAS-ELISA as a sole YMV certification method since the detecting monoclonal antibody used in this assay may be strain specific. The use of PAS-ELISA at a 1/10 sap dilution is suggested for YMV detection where the facilities for molecular detection are unavailable.
dc.identifierhttps://hdl.handle.net/10568/79829
dc.identifier.urihttp://hdl.handle.net/123456789/123052
dc.languageen
dc.publisherInternational Digital Organization for Scientific Information (IDOSI)
dc.rightsLimited Access
dc.sourceEni, A., Hughes, J., Asiedu, R., & Rey, M. (2012). Re-evaluation of yam mosaic virus (YMV) detection methods. Academic Journal of Plant Sciences, 5(1), 18-22.
dc.subjectyam mosaic virus
dc.subjectdetection sensitivity
dc.subjectgenus potyvirus
dc.subjectyams
dc.subjectdioscorea
dc.subjectepidemiological
dc.subjectisolation
dc.subjectalkaline phosphatase
dc.titleRe-evaluation of Yam Mosaic Virus (YMV) detection methods
dc.typeJournal Article

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Instituto Internacional de Agricultura Tropical (IITA)