Ability of a Lactobacillus rhamnosus strain cultured in milk whey based medium to bind aflatoxin B1

Fernanda  BOVO; Larissa  Tuanny FRANCO; Roice Eliana  ROSIM; Carlos Augusto  Fernandes de OLIVEIRA

Ability of a Lactobacillus rhamnosus strain cultured in milk whey based medium to bind aflatoxin B1

Autores

Fernanda BOVO

Larissa Tuanny FRANCO

Roice Eliana ROSIM

Carlos Augusto Fernandes de OLIVEIRA

Editor

Sociedade Brasileira de Ciência e Tecnologia de Alimentos

Descripción

This study aimed to compare Lactobacillus rhamnosus growth in MRS (de Man, Rogosa and Sharpe) broth and a culture medium containing milk whey (MMW) and to evaluate aflatoxin B 1 (AFB 1 ) adsorption capacity by bacterial cells produced in both culture media. L. rhamnosus cells were cultivated in MRS broth and MMW (37 °C, 24 hours), and bacterial cell concentration was determined spectrophotometrically at 600 nm. AFB 1 (1 μg/ml) adsorption assays were conducted using 1 x 10 10 non-viable L. rhamnosus cells (121 °C, 15 minutes) at pHs 3.0 and 6.0 and contact time of 60 minutes. AFB 1 quantification was performed by High Performance Liquid Chromatography. Bacterial cell concentration in MMW was higher (9.84 log CFU/ml) than that in MRS broth (9.63 log CFU/ml). There were no significant differences between AFB 1 binding results at the same pH value (3.0 or 6.0) for the cells cultivated in MRS broth (46.0% and 35.8%, respectively) and in MMW (43.7% and 25.8%, respectively), showing that MMW can adequately replace the MRS broth. Therefore, it can be concluded that the use of L. rhamnosus cells cultivated in MMW offers advantages such as reduction in large scale production costs, improvement of environmental sustainability, and being a practicable alternative for decontamination of food products susceptible to aflatoxin contamination.