Identification and classification of Mycobacterium by MALDI-TOF mass spectrometry.

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dc.contributorLARISSA COSTA OLARTE, UFMS - Federal University of Mato Grosso do Sul (Campo Grande - MS - Brasil); LARISSA DE FÁTIMA CARDOSO DUARTE, UFMS - Federal University of Mato Grosso do Sul (Campo Grande - MS - Brasil); GISELE OLIVAS DE CAMPOS LEGUIZAMON, CNPGC; FLABIO RIBEIRO ARAUJO, CNPGC; NEWTON VALERIO VERBISCK, CNPGC.
dc.creatorOLARTE, L. C.
dc.creatorDUARTE, L. de F. C.
dc.creatorLEGUIZAMON, G. O. de C.
dc.creatorARAUJO, F. R.
dc.creatorVERBISCK, N. V.
dc.date2017-11-10T23:25:22Z
dc.date2017-11-10T23:25:22Z
dc.date2017-11-10
dc.date2016
dc.date2017-11-13T11:11:11Z
dc.date.accessioned2026-07-07T03:40:02Z
dc.descriptionBovine tuberculosis is a zoonotic disease caused by the Mycobacterium bovis bacillum, which accounts for significant economic losses in cattle production besides being a public health threat. Our purpose is to establish MALDI-TOF (Matrix Assisted Laser Desorption Ionization –Time-of-Flight) mass spectrometry method to identify and classify mycobacteria species, by means of building a specific biomarker library after solid media culture for these microrganims. We have identified 26 different protocols employing MALDI-TOF to identify and classify mycobacteria from solid media culture within 58 articles published since 2010. Four consensus protocols were designed and our results lead to the following cell treatment conditions before MALDI-TOF analysis: heat inactivation at 95ºC for 45 minutes, disruption with zirconia beads and MagNA lyser (Roche) and formic acid 70% and acetonitrile protein extraction.
dc.identifierIn: IBERO-AMERICAN, 1.; BrMASS CONFERENCE ON MASS SPECTROMETRY, 6., 2016, Rio de Janeiro. Resumos... Campinas: Sociedade Brasileira de Espectrometria de Massas, 2016.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1079459
dc.identifier.urihttp://hdl.handle.net/123456789/440193
dc.languageeng
dc.rightsopenAccess
dc.subjectMALDI-TOF mass spectrometry
dc.subjectMycobacterium Bovis
dc.subjectMycobacterium
dc.subjectBovine tuberculosis
dc.subjectSpecies identification
dc.subjectMycobacterium smegmatis
dc.titleIdentification and classification of Mycobacterium by MALDI-TOF mass spectrometry.
dc.typeResumo em anais e proceedings

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