Identification of coagulase negative staphylococci isolated from bovine mastitis by 16S rDNA sequencing.

LANGE, C. C.; BRITO, M. A. V. P. e; SILVA, F. S.; ALVIM, M. C. T.; REIS, D. R. de L.; DOMINGUES, R.

Identification of coagulase negative staphylococci isolated from bovine mastitis by 16S rDNA sequencing.

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Descripción

Mastitis is a multifactorial disease, which often has a bacterial onçm, and affects cattle worldwide. The genus Staphylococcus contains at least 40 species and 17 subspecies and, under certain circumstances, many of these species can cause mastitis. In herds that have achieved control of infectious agents, especially Staphylococcus aureus and Streptococcus agalactiae, other Staphylococcus species have often been associated with mastitis in cattle. In this study a total of 63 coagulase-negative staphylococci (CNS) were subjected to sequencing of the 16S rDNA to species identification. The sequencing reactions were done on the MegaBACE 1000 DNA sequencer (GE Healthcare, NYSE, Germany).