Genetic diversity of the common bean Phaseolus vulgaris L. determined by DNA-based molecular markers.

VASCONCELOS, M. J. V.; BARROS, E. G. de; MOREIRA, M. A.; VIEIRA, C.

Genetic diversity of the common bean Phaseolus vulgaris L. determined by DNA-based molecular markers.

Autores

VASCONCELOS, M. J. V.

BARROS, E. G. de

MOREIRA, M. A.

VIEIRA, C.

Descripción

Weselected 28 potential common bean (Phaseolus vulgaris L.) progenitors to determine their genetic diversity using RAPD technique. They were divided into two groups based on their type of storage protein (phaseolin); 18 of them had type "S" phaseolin and the other 10 had phaseolin. DNA samples of all the cultivars were amplified in vitro with oligonucleotide primiers of random sequences. A total of 276 amplification products, 144 of them being polymorphic, were obtained with 45 different primers. Multivariate analysis of these data divided the cultivars into groups and, in addition, allowed a more precise intra and intergroup distinction.