Inorganic nitrogen application affects both taxonomical and predicted functional structure of wheat rhizosphere bacterial communities.

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dc.contributorVANESSA NESSNER KAVAMURA, Rothamsted Research; RIFAT HAYAT, PMAS Arid Agriculture University; IAN MICHAEL CLARK, Rothamsted Research; MAIKE ROSSMANN, FAPESP; RODRIGO MENDES, CNPMA; PENNY R HIRSCH, Rothamsted Research; TIM H MAUCHLINE, Rothamsted Research.
dc.creatorKAVAMURA, V. N.
dc.creatorHAYAT, R.
dc.creatorCLARK, I. M.
dc.creatorROSSMANN, M.
dc.creatorMENDES, R.
dc.creatorHIRSCH, P. R.
dc.creatorMAUCHLINE, T. H.
dc.date2019-02-04T23:39:12Z
dc.date2019-02-04T23:39:12Z
dc.date2019-02-04
dc.date2018
dc.date2019-02-15T11:11:11Z
dc.date.accessioned2026-07-07T05:06:43Z
dc.descriptionThe effects of fertilizer regime on bulk soil microbial communities have been well studied, but this is not the case for the rhizosphere microbiome. The aim of this work was to assess the impact of fertilization regime on wheat rhizosphere microbiome assembly and 16S rRNA gene-predicted functions with soil from the long term Broadbalk experiment at Rothamsted Research. Soil from four N fertilization regimes (organic N, zero N, medium inorganic N and high inorganic N) was sown with seeds of Triticum aestivum cv. Cadenza. 16S rRNA gene amplicon sequencing was performed with the Illumina platform on bulk soil and rhizosphere samples of 4-week-old and flowering plants (10 weeks). Phylogenetic and 16S rRNA gene-predicted functional analyses were performed. Fertilization regime affected the structure and composition of wheat rhizosphere bacterial communities. Acidobacteria and Planctomycetes were significantly depleted in treatments receiving inorganic N, whereas the addition of high levels of inorganic N enriched members of the phylum Bacteroidetes, especially after 10 weeks. Bacterial richness and diversity decreased with inorganic nitrogen inputs and was highest after organic treatment (FYM). In general, high levels of inorganic nitrogen fertilizers negatively affect bacterial richness and diversity, leading to a less stable bacterial community structure over time, whereas, more stable bacterial communities are provided by organic amendments. 16S rRNA gene-predicted functional structure was more affected by growth stage than by fertilizer treatment, although, some functions related to energy metabolism and metabolism of terpenoids and polyketides were enriched in samples not receiving any inorganic N, whereas inorganic N addition enriched predicted functions related to metabolism of other amino acids and carbohydrates. Understanding the impact of different fertilizers on the structure and dynamics of the rhizosphere microbiome is an important step toward developing strategies for production of crops in a sustainable way.
dc.format15 p.
dc.identifierFrontiers in Microbiology, Lausanne, v. 9, 2018. Article 1074.
dc.identifier1664-302X
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1105404
dc.identifierhttps://doi.org/10.3389/fmicb.2018.01074
dc.identifier.urihttp://hdl.handle.net/123456789/480710
dc.languageeng
dc.rightsopenAccess
dc.subjectNitrogênio inorgânico
dc.subjectTrigo
dc.subjectFertilizante nitrogenado
dc.subjectImpacto ambiental
dc.subjectRizosfera
dc.subjectWheat
dc.subjectRhizosphere
dc.subjectMicrobiome
dc.subjectNitrogen fertilizers
dc.subjectEnvironmental impact
dc.subjectBacterial communities
dc.subjectFunctional diversity
dc.subjectSustainable agricultural intensification
dc.titleInorganic nitrogen application affects both taxonomical and predicted functional structure of wheat rhizosphere bacterial communities.
dc.typeArtigo de periódico

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