Broad-spectrum resistance to bacterial blight in rice using genome editing

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dc.creatorOliva, Ricardo
dc.creatorJi, Chonghui
dc.creatorAtienza-Grande, Genelou
dc.creatorHuguet-Tapia, José C.
dc.creatorPerez-Quintero, Alvaro
dc.creatorLi, Ting
dc.creatorEom, Joon-Seob
dc.creatorLi, Chenhao
dc.creatorNguyen, Hanna
dc.creatorLiu, Bo
dc.creatorAuguy, Florence
dc.creatorSciallano, Coline
dc.creatorLuu, Van T.
dc.creatorDossa, Gerbert S.
dc.creatorCunnac, Sébastien
dc.creatorSchmidt, Sarah M.
dc.creatorSlamet-Loedin, Inez H.
dc.creatorVera Cruz, Casiana
dc.creatorSzurek, Boris
dc.creatorFrommer, Wolf B.
dc.creatorWhite, Frank F.
dc.creatorYang, Bing
dc.date2019-11
dc.date2024-12-19T12:54:06Z
dc.date2024-12-19T12:54:06Z
dc.date.accessioned2026-06-27T04:07:37Z
dc.descriptionBacterial blight of rice is an important disease in Asia and Africa. The pathogen,Xanthomonas oryzaepv.oryzae(Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transporter genesSWEET11,SWEET13andSWEET14, the expression of which is required for disease susceptibility. We used CRISPR–Cas9-mediated genome editing to introduce mutations in all threeSWEETgene promoters. Editing was further informed by sequence analyses of TALe genes in 63Xoostrains, which revealed multiple TALe variants forSWEET13alleles. Mutations were also created inSWEET14, which is also targeted by two TALes from an AfricanXoolineage. A total of five promoter mutations were simultaneously introduced into the rice line Kitaake and the elite mega varieties IR64 and Ciherang-Sub1. Paddy trials showed that genome-editedSWEETpromoters endow rice lines with robust, broad-spectrum resistance.
dc.identifierhttps://hdl.handle.net/10568/164605
dc.identifier.urihttp://hdl.handle.net/123456789/22403
dc.languageen
dc.publisherSpringer
dc.rightsOpen Access
dc.sourceOliva, Ricardo; Ji, Chonghui; Atienza-Grande, Genelou; Huguet-Tapia, José C.; Perez-Quintero, Alvaro; Li, Ting; Eom, Joon-Seob; Li, Chenhao; Nguyen, Hanna; Liu, Bo; Auguy, Florence; Sciallano, Coline; Luu, Van T.; Dossa, Gerbert S.; Cunnac, Sébastien; Schmidt, Sarah M.; Slamet-Loedin, Inez H.; Vera Cruz, Casiana; Szurek, Boris; Frommer, Wolf B.; White, Frank F. and Yang, Bing. 2019. Broad-spectrum resistance to bacterial blight in rice using genome editing. Nat Biotechnol, Volume 37 no. 11 p. 1344-1350
dc.subjectapplied microbiology and biotechnology
dc.subjectbioengineering
dc.subjectbiomedical engineering
dc.subjectbiotechnology
dc.subjectmolecular medicine
dc.titleBroad-spectrum resistance to bacterial blight in rice using genome editing
dc.typeJournal Article

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Instituto Internacional de Investigación del Arroz (IRRI)