Effect of replacer of fetal calf serum in the development and gene expression in bovine embryos in vitro cultured.
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The period of post fertilization embryo culture is the most critical affecting blastocyst quality. Knockout SR (Gibco Labs., Grand Island, NY) is a serum replacer optimized to support embryonic stem cells in culture and can also be used to replace serum during culture of bovine embryos. The expression of genes associated to stress response, such as heat shock proteins (Hsp), can be affected by in vitro culture conditions, including culture medium components. The aim of this study was to evaluate the effect of KnockoutTM SR on the development, total number cells and relative abundance of Hsp70.1 of in vitro fertilized bovine embryos.
Edição dos abstracts do 16º International Congress on Animal Reproduction, Budapest, 2008.
Edição dos abstracts do 16º International Congress on Animal Reproduction, Budapest, 2008.
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Embrião bovino