AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis

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dc.creatorRestrepo, S.
dc.creatorDuque E., Myriam Cristina
dc.creatorTohme, Joseph M.
dc.creatorVerdier, Valerie M.
dc.date1999
dc.date2014-09-24T07:58:12Z
dc.date2014-09-24T07:58:12Z
dc.date.accessioned2026-06-27T14:42:56Z
dc.descriptionSummary: Xanthomonas axonopodis pv. manihotis (Xam) is the causative agent of cassava bacterial blight (CBB), a worldwide disease that is particularly destructive in South America and Africa. CBB is controlled essentially through the use of resistant varieties. To develop an appropriate disease management strategy, the genetic diversity of the pathogen's populations must be assessed. Until now, the genetic diversity of Xam was characterized by RFLP analyses using ribotyping, and plasmid and genomic Xam probes. We used AFLP (amplified fragment length polymorphism), a novel PCR-based technique, to characterize the genetic diversity of Colombian Xam isolates. Six Xam strains were tested with 65 AFLP primer combinations to identify the best selective primers. Eight primer combinations were selected according to their reproducibility, number of polymorphic bands and polymorphism detected between Xam strains. Forty-seven Xam strains, originating from different Colombian ecozones, were analysed with the selected combinations. Results obtained with AFLP are consistent with those obtained with RFLP, using plasmid DNA as a probe. Some primer combinations differentiated Xam strains that were not distinguished by RFLP analyses, thus AFLP fingerprinting allowed a better definition of the genetic relationships between Xam strains.
dc.identifierhttps://hdl.handle.net/10568/42558
dc.identifier.urihttp://hdl.handle.net/123456789/86261
dc.languageen
dc.rightsOpen Access
dc.subjectmanihot esculenta
dc.subjectxanthomonas axonopodis
dc.subjectgenetic markers
dc.subjectdna fingerprinting
dc.subjectdna
dc.subjectgenetic variation
dc.subjectmarcadores genéticos
dc.subjecthuellas genéticas adn
dc.subjectadn
dc.subjectvariación genética
dc.titleAFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis
dc.typeJournal Article

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Centro Internacional de Agricultura Tropical (CIAT)