Interaction transcriptome analysis identifies Magnaporthe oryzae BAS1-4 as biotrophy-associated secreted proteins in rice blast disease

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dc.creatorMosquera Cifuentes, Gloria Maria
dc.creatorGiraldo, M.C.
dc.creatorKhang, CH
dc.creatorCoughlan, S
dc.creatorValent, B
dc.date2009-05-22
dc.date2014-09-24T08:42:23Z
dc.date2014-09-24T08:42:23Z
dc.date.accessioned2026-06-27T14:46:48Z
dc.descriptionBiotrophic invasive hyphae (IH) of the blast fungus Magnaporthe oryzae secrete effectors to alter host defenses and cellular processes as they successively invade living rice (Oryza sativa) cells. However, few blast effectors have been identified. Indeed, understanding fungal and rice genes contributing to biotrophic invasion has been difficult because so few plant cells have encountered IH at the earliest infection stages. We developed a robust procedure for isolating infected-rice sheath RNAs in which ;20% of the RNA originated from IH in first-invaded cells. We analyzed these IH RNAs relative to control mycelial RNAs using M. oryzae oligoarrays. With a 10-fold differential expression threshold, we identified known effector PWL2 and 58 candidate effectors. Four of these candidates were confirmed to be fungal biotrophy-associated secreted (BAS) proteins. Fluorescently labeled BAS proteins were secreted into rice cells in distinct patterns in compatible, but not in incompatible, interactions. BAS1 and BAS2 proteins preferentially accumulated in biotrophic interfacial complexes along with known avirulence effectors, BAS3 showed additional localization near cell wall crossing points, and BAS4 uniformly outlined growing IH. Analysis of the same infected-tissue RNAs with rice oligoarrays identified putative effector-induced rice susceptibility genes, which are highly enriched for sensor-transduction components rather than typically identified defense response genes.
dc.identifierhttps://hdl.handle.net/10568/43605
dc.identifier.urihttp://hdl.handle.net/123456789/88267
dc.languageen
dc.publisherOxford University Press
dc.rightsOpen Access
dc.subjectoryza sativa
dc.subjectrice
dc.subjectrna
dc.subjectfluorescence
dc.subjectblights
dc.subjectplant diseases
dc.subjectmagnaporthe grisea
dc.subjectpyricularia grisea
dc.subjectarroz
dc.subjectarn
dc.subjectfluorescencia
dc.subjecttizón
dc.subjectenfermedades de las plantas
dc.titleInteraction transcriptome analysis identifies Magnaporthe oryzae BAS1-4 as biotrophy-associated secreted proteins in rice blast disease
dc.typeJournal Article

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Centro Internacional de Agricultura Tropical (CIAT)